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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/27688
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dc.contributor.authorCicirò, Y-
dc.contributor.authorRagusa, D-
dc.contributor.authorNevado, PT-
dc.contributor.authorLattanzio, R-
dc.contributor.authorSala, G-
dc.contributor.authorDes Rochers, T-
dc.contributor.authorMillard, M-
dc.contributor.authorAndersson, M-
dc.contributor.authorStenman, G-
dc.contributor.authorSala, A-
dc.date.accessioned2023-11-21T00:30:40Z-
dc.date.available2023-11-21T00:30:40Z-
dc.date.issued2023-12-19-
dc.identifierORCID iD: Denise Ragusa https://orcid.org/0000-0002-0303-8683-
dc.identifierORCID iD: Gianluca Sala https://orcid.org/0000-0002-4494-915X-
dc.identifierORCID iD: Mattias K. Andersson https://orcid.org/0000-0001-6391-2048-
dc.identifierORCID iD: Arturo Sala https://orcid.org/0000-0002-2841-7866-
dc.identifier.citationCicirò, Y et al. (2024) 'The mitotic checkpoint kinase BUB1 is a direct and actionable target of MYB in adenoid cystic carcinoma', FEBS Letters, 598 (2), pp. 252 - 265. doi: 10.1111/andr.13582.en_US
dc.identifier.urihttps://bura.brunel.ac.uk/handle/2438/27688-
dc.descriptionData accessibility: ChIP-seq data on ACCX11 cells are available at ArrayExpress accession E-MTAB-12976. Microarray datasets were downloaded from the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) repository. The Andersson dataset (GEO accession number GSE88804) reported expression data of 13 surgical samples of ACC and 7 NSG samples [[8]]. The Chowbina dataset (GEO accession number GSE36820) presented microarray analysis on 3 normal samples and 11 ACC xenograft samples. The Gao dataset (GEO accession number GSE59702) contains expression profiling by array of 12 ACC with matched normal tissues [[9]]. RNAseq data from Cicirò dataset report 7 replicates of ACC samples (ACCX11) and 3 NSG samples; data available at ArrayExpress accession E-MTAB-12978. RNAseq data of MM MYB/EV ± DOX are available at ArrayExpress accession E-MTAB-12977.en_US
dc.descriptionSupporting Information is available onloine at: https://febs.onlinelibrary.wiley.com/doi/full/10.1002/1873-3468.14786#support-information-section .-
dc.description.abstractCopyright © 2023 The Authors. Adenoid cystic carcinoma (ACC) is a head and neck cancer that frequently originates in salivary glands, but can also strike other exocrine glands such as the breast. A key molecular alteration found in the majority of ACC cases is MYB gene rearrangements, leading to activation of the oncogenic transcription factor MYB. In this study, we used immortalised breast epithelial cells and an inducible MYB transgene as a model of ACC. Molecular profiling confirmed that MYB-driven gene expression causes a transition into an ACC-like state. Using this new cell model, we identified BUB1 as a targetable kinase directly controlled by MYB, whose pharmacological inhibition caused MYB-dependent synthetic lethality, growth arrest and apoptosis of patient-derived cells and organoids.-
dc.description.sponsorshipThis research was supported by grants from the Oracle Cancer Trust and ACCRF to AS, Fondazione-AIRC (IG 2021 id 25696) to GS and the Swedish Cancer Society and the Sjöberg Foundation to GS and MKA. DR is supported by the BBSRC grant BB/V013920/1 to Prof Paola Vagnarelli (Brunel University London).en_US
dc.format.extent252 - 265-
dc.format.mediumPrint-Electronic-
dc.language.isoen_USen_US
dc.publisherWiley on behalf of Federation of European Biochemical Societiesen_US
dc.rightsCopyright © 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectadenoid cystic carcinomaen_US
dc.subjecthead and neck canceren_US
dc.subjectMYBen_US
dc.subjectBUB1en_US
dc.subject3D assayen_US
dc.subjectBAY1816032en_US
dc.subjectPDXen_US
dc.subjectRNA sequencingen_US
dc.subjectChIP sequencingen_US
dc.titleThe mitotic checkpoint kinase BUB1 is a direct and actionable target of MYB in adenoid cystic carcinomaen_US
dc.title.alternativeIdentification of the mitotic checkpoint kinase BUB1 is a direct and actionable target of MYB in adenoid cystic carcinoma-
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1111/andr.13582-
dc.relation.isPartOfFEBS Letters-
pubs.issue2-
pubs.publication-statusPublished-
pubs.volume598-
dc.rights.holderThe Authors-
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