Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/26160
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dc.contributor.authorKandzija, N-
dc.contributor.authorRahbar, M-
dc.contributor.authorJones, GD-
dc.contributor.authorMotta-Mejia, C-
dc.contributor.authorZhang, W-
dc.contributor.authorCouch, Y-
dc.contributor.authorNeuhaus, AA-
dc.contributor.authorKishore, U-
dc.contributor.authorSutherland, BA-
dc.contributor.authorRedman, C-
dc.contributor.authorVatish, M-
dc.date.accessioned2023-03-19T13:59:48Z-
dc.date.available2023-03-19T13:59:48Z-
dc.date.issued2023-02-07-
dc.identifierORCID iDs: Neva Kandzija https://orcid.org/0000-0003-0943-6049; Carolina Motta-Mejia https://orcid.org/0000-0002-1976-177X; Wei Zhang https://orcid.org/0000-0002-4391-2523; Ain A. Neuhaus https://orcid.org/0000-0003-2177-2387; Uday Kishore https://orcid.org/0000-0002-6033-6759-
dc.identifier.citationKandzija , N. et al. (2023) 'Placental capillary pericytes release excess extracellular vesicles under hypoxic conditions inducing a pro-angiogenic profile in term pregnancy', Biochemical and Biophysical Research Communications, 651, pp. 20 - 29. doi: 10.1016/j.bbrc.2023.02.015.en_US
dc.identifier.issn0006-291X-
dc.identifier.urihttps://bura.brunel.ac.uk/handle/2438/26160-
dc.descriptionSupplementary data available online at: https://www.sciencedirect.com/science/article/pii/S0006291X2300181X#appsec1en_US
dc.description.abstractCopyright © 2023 The Authors. Pericytes are multifunctional cells wrapped around capillary endothelia, essential for vascular health, development, and blood flow regulation, although their role in human placental chorionic villi has not been fully explored. The second half of normal pregnancy is characterized by a progressive decline in placental and fetal oxygen levels which, by term, comprises a substantial degree of hypoxia. We hypothesized this hypoxia would stimulate pericyte regulation of chorionic villous capillary function. This study's objective was to investigate the role of hypoxia on normal term placental pericytes (PLVP) and their signaling to endothelial cells. First, we confirmed fetoplacental hypoxia at term by a new analysis of umbilical arterial blood oxygen tension of 3,010 healthy singleton neonates sampled at caesarean section and before labor. We then measured the release of cytokines, chemokines, and small extracellular vesicles (PLVPsv), from PLVP cultured at 20%, 8% and 1% O2. As O2 levels decreased, secreted cytokines and chemokines [interleukin-6 (IL-6), interleukin-1α (IL-1α) and vascular endothelial growth factor (VEGF)], and small extracellular vesicle markers, (Alix, Syntenin and CD9) increased significantly in the culture supernatants. When primary human umbilical vein endothelial cells (HUVEC) were cultured with PLVPsv, polygon formation, number, and tube formation length was significantly increased compared to cells not treated with PLVPsv, indicating PLVPsv stimulated angiogenesis. We conclude that adding PLVPsv stimulates angiogenesis and vessel stabilization on neighboring endothelial cells in response to hypoxia in term pregnancy compared to no addition of PLVPsv. Our finding that PLVP can release angiogenic molecules via extracellular vesicles in response to hypoxia may apply to other organ systems.en_US
dc.description.sponsorshipMRC Program Grant (MR/J003360/1); rad Sutherland is supported by the National Health and Medical Research Council of Australia (APP1137776).en_US
dc.format.extent20 - 29-
dc.languageEnglish-
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.rightsCopyright © 2023 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectplacental pericytesen_US
dc.subjecthypoxiaen_US
dc.subjectsmall extracellular vesiclesen_US
dc.subjectangiogenesisen_US
dc.titlePlacental capillary pericytes release excess extracellular vesicles under hypoxic conditions inducing a pro-angiogenic profile in term pregnancyen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.bbrc.2023.02.015-
dc.relation.isPartOfBiochemical and Biophysical Research Communications-
pubs.publication-statusPublished-
pubs.volume651-
dc.rights.holderThe Authors-
Appears in Collections:Dept of Life Sciences Research Papers

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