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DC Field | Value | Language |
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dc.contributor.author | Stamatiou, K | - |
dc.contributor.author | Chmielewska, A | - |
dc.contributor.author | Ohta, S | - |
dc.contributor.author | Earnshaw, WC | - |
dc.contributor.author | Vagnarelli, P | - |
dc.date.accessioned | 2023-01-17T16:06:09Z | - |
dc.date.available | 2023-01-17T16:06:09Z | - |
dc.date.issued | 2023-01-25 | - |
dc.identifier | ORCiD: Konstantinos Stamatiou https://orcid.org/0000-0002-5650-078X | - |
dc.identifier | ORCiD: Aldona Chmielewska https://orcid.org/0000-0003-3411-6776 | - |
dc.identifier | ORCiD: Shinya Ohta https://orcid.org/0000-0003-2424-1741 | - |
dc.identifier | ORCiD: William C. Earnshaw https://orcid.org/0000-0002-7191-0621 | - |
dc.identifier | ORCiD: Paola Vagnarelli https://orcid.org/0000-0002-0000-2271 | - |
dc.identifier.citation | Stamatiou, K. et al. (2023) 'CCDC86 is a novel Ki-67-interacting protein important for cell division', Journal of Cell Science, 136 (2), pp. 1 - 13. doi: 10.1242/jcs.260391. | en_US |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/25800 | - |
dc.description | Data availability: Imaging and quantification datasets are available upon request from the corresponding authors. | en_US |
dc.description | Acknowledgements: We would like to acknowledge Prof. Arturo Sala (Brunel University London) for useful discussion on the link between CCDC86 and MYCN. | - |
dc.description | Supplementary information is available online at: https://journals.biologists.com/jcs/article/136/2/jcs260391/286751/CCDC86-is-a-novel-Ki-67-interacting-protein#supplementary-data . | - |
dc.description.abstract | The chromosome periphery is a network of proteins and RNAs that coats the outer surface of mitotic chromosomes. Despite the identification of new components, the functions of this complex compartment are poorly characterised. In this study, we identified a novel chromosome periphery-associated protein, CCDC86 (also known as cyclon). Using a combination of RNA interference, microscopy and biochemistry, we studied the functions of CCDC86 in mitosis. CCDC86 depletion resulted in partial disorganisation of the chromosome periphery with alterations in the localisation of Ki-67 (also known as MKI67) and nucleolin (NCL), and the formation of abnormal cytoplasmic aggregates. Furthermore, CCDC86-depleted cells displayed errors in chromosome alignment, altered spindle length and increased apoptosis. These results suggest that, within the chromosome periphery, different subcomplexes that include CCDC86, nucleolin and B23 (nucleophosmin or NPM1) are required for mitotic spindle regulation and correct kinetochore–microtubule attachments, thus contributing to chromosome segregation in mitosis. Moreover, we identified CCDC86 as a MYCN-regulated gene, the expression levels of which represent a powerful marker for prognostic outcomes in neuroblastoma. | en_US |
dc.description.sponsorship | The Vagnarelli laboratory is supported by the Wellcome Trust Investigator award 210742/Z/18/Z to P.V. The study on MYC (Fig. 5) was supported by a Kidscan Children's Cancer Research PhD studentship awarded to P.V. The Ohta laboratory is supported by Research Foundation for the Electrotechnology of Chubu (grant R-01227) to S.O. Work in the Earnshaw laboratory is funded by Wellcome, from whom W.C.E. holds a Principal Research Fellowship (grant 107022). Open Access funding provided by Brunel University London. Deposited in PMC for immediate release. | en_US |
dc.format.extent | 1 - 13 | - |
dc.format.medium | Print-Electronic | - |
dc.language.iso | en | en_US |
dc.publisher | The Company of Biologists | en_US |
dc.rights | Copyright © 2023. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | cell division | - |
dc.subject | chromosomes | - |
dc.subject | MYCN | - |
dc.subject | CCDC86 | - |
dc.subject | Ki-67 | - |
dc.title | CCDC86 is a novel Ki-67-interacting protein important for cell division | en_US |
dc.title.alternative | CCDC86/Cyclon is a novel Ki-67 interacting protein important for cell division | - |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1242/jcs.260391 | - |
dc.relation.isPartOf | Journal of Cell Science | - |
pubs.issue | 2 | - |
pubs.publication-status | Published | - |
pubs.volume | 136 | - |
dc.identifier.eissn | 1477-9137 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/legalcode.en | - |
dc.rights.holder | The Author(s) / The Company of Biologists | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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