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DC Field | Value | Language |
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dc.contributor.author | Alshaya, DS | - |
dc.contributor.author | Jalal, AS | - |
dc.contributor.author | Alburae, NA | - |
dc.contributor.author | Aljarba, NH | - |
dc.contributor.author | Murugaiah, V | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Al-Qahtani, AA | - |
dc.date.accessioned | 2022-03-04T10:13:48Z | - |
dc.date.available | 2022-03-04T10:13:48Z | - |
dc.date.issued | 2022-02-07 | - |
dc.identifier | 101851 | - |
dc.identifier.citation | Alshaya, D.S., Jalal, A.S., Alburae, N.A., Aljarba, N.H., Murugaiah, V., Kishore, U. and Al-Qahtani, A.A. (2022) 'Carbon nanotube-coated recombinant human surfactant protein D reduces cell viability in an ovarian cancer cell line, SKOV3, and modulates mTOR pathway and pro-inflammatory cytokine response', Journal of King Saud University - Science, 34 (3), 101851, pp. 1 - 7 (7). doi: 10.1016/j.jksus.2022.101851. | en_US |
dc.identifier.issn | 1018-3647 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/24203 | - |
dc.description.abstract | Copyright © 2022 The Authors. Nanoparticles such as carbon nanotubes (CNTs) have various clinical and diagnostic applications as utilised for imaging and drug delivery. Therapeutic proteins/peptides can be loaded on CNT coronas to specifically hit immune cells in overdrive or uncontrolled malignant cells. Previously, it was reported that a recombinant version of human surfactant protein D (rfhSP-D) containing trimeric C-type lectin domains induced apoptosis in several tumour cells/cell lines, including SKOV3, which is an ovarian tumour cell line. Solid-phase rfhSP-D coated on a microtiter plate is considerably more potent in inducing apoptosis in breast tumour cells. We have immobilised highly purified, endotoxin-free rfhSP-D on CNTs and assessed its antiproliferative effect on SKOV3 cells. Biotinylated rfhSP-D-CNTs were phagocytosed by SKOV3 cells, followed by apoptosis in a time-dependent manner. Gene expression analysis revealed compromised mTOR complex as a mechanism of apoptosis. When rfhSP-D-CNTs were added to a culture system of SKOV3 cells, it produced a highly proinflammatory immune response that is likely anti-tumorigenic. Thus, rfhSP-D-CNT seems a worthwhile nanocarrier for testing in vivo using an animal model of orthotopic ovarian cancer. | en_US |
dc.description.sponsorship | This research was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, Riyadh, KSA, through the Research Funding Program (Grant No# FRP-1440-27). | en_US |
dc.format.extent | 1 - 7 (7) | - |
dc.format.medium | Print-Electronic | - |
dc.language | Enlish | - |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier BV on behalf of King Saud University. | en_US |
dc.rights | Copyright © 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | carbon nanotubes | en_US |
dc.subject | rfhSP-D | en_US |
dc.subject | drug delivery | en_US |
dc.subject | SKOV3 | en_US |
dc.subject | anti-tumorigenic | en_US |
dc.subject | ovarian cancer | en_US |
dc.title | Carbon nanotube-coated recombinant human surfactant protein D reduces cell viability in an ovarian cancer cell line, SKOV3, and modulates mTOR pathway and pro-inflammatory cytokine response | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.jksus.2022.101851 | - |
dc.relation.isPartOf | Journal of King Saud University - Science | - |
pubs.issue | 3 | - |
pubs.publication-status | Published online | - |
pubs.volume | 34 | - |
dc.identifier.eissn | 2213-686X | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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