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DC Field | Value | Language |
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dc.contributor.author | Khan, HA | - |
dc.contributor.author | Kishore, U | - |
dc.contributor.author | Alsulami, HM | - |
dc.contributor.author | Alrokayan, SH | - |
dc.date.accessioned | 2022-03-03T17:24:41Z | - |
dc.date.available | 2022-03-03T17:24:41Z | - |
dc.date.issued | 2021-09-28 | - |
dc.identifier | 10445 | - |
dc.identifier.citation | Khan, H.A., Kishore, U., Alsulami, H.M. and Alrokayan, S.H. (2021) ‘Pro-Apoptotic and Immunotherapeutic Effects of Carbon Nanotubes Functionalized with Recombinant Human Surfactant Protein D on Leukemic Cells’, International Journal of Molecular Sciences, 22 (19), 10445, pp. 1 - 15. doi: 10.3390/ijms221910445. | en_US |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/24202 | - |
dc.description | Data Availability Statement: Not applicable. | en_US |
dc.description.abstract | Copyright: © 2021 by the authors. Nanoparticles are efficient drug delivery vehicles for targeting specific organs as well as systemic therapy for a range of diseases, including cancer. However, their interaction with the immune system offers an intriguing challenge. Due to the unique physico-chemical properties, carbon nanotubes (CNTs) are considered as nanocarriers of considerable interest in cancer diagnosis and therapy. CNTs, as a promising nanomaterial, are capable of both detecting as well as delivering drugs or small therapeutic molecules to tumour cells. In this study, we coupled a recombinant fragment of human surfactant protein D (rfhSP-D) with carboxymethyl-cellulose (CMC) CNTs (CMC-CNT, 10–20 nm diameter) for augmenting their apoptotic and immunotherapeutic properties using two leukemic cell lines. The cell viability of AML14.3D10 or K562 cancer cell lines was reduced when cultured with CMC-mwCNT-coupled-rfhSP-D (CNT + rfhSP-D) at 24 h. Increased levels of caspase 3, 7 and cleaved caspase 9 in CNT + rfhSP-D treated AML14.3D10 and K562 cells suggested an involvement of an intrinsic pathway of apoptosis. CNT + rfhSP-D treated leukemic cells also showed higher mRNA expression of p53 and cell cycle inhibitors (p21 and p27). This suggested a likely reduction in cdc2-cyclin B1, causing G2/M cell cycle arrest and p53-dependent apoptosis in AML14.3D10 cells, while p53-independent mechanisms appeared to be in operation in K562 cells. We suggest that CNT + rfhSP-D has therapeutic potential in targeting leukemic cells, irrespective of their p53 status, and thus, it is worth setting up pre-clinical trials in animal models | en_US |
dc.description.sponsorship | Funding: This project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia, Award Number (15-NAN-3664-02). | en_US |
dc.format.extent | 1 - 15 | - |
dc.format.medium | Print-Electronic | - |
dc.language | English | - |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI AG | en_US |
dc.rights | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | carbon nanotubes | en_US |
dc.subject | human SP-D | en_US |
dc.subject | cancer cells | en_US |
dc.subject | apoptosis | en_US |
dc.subject | immunotherapy | en_US |
dc.title | Pro-apoptotic and immunotherapeutic effects of carbon nanotubes functionalized with recombinant human surfactant protein d on leukemic cells | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms221910445 | - |
dc.relation.isPartOf | International Journal of Molecular Sciences | - |
pubs.issue | 19 | - |
pubs.publication-status | Published | - |
pubs.volume | 22 | - |
dc.identifier.eissn | 1422-0067 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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