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Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/23953
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dc.contributor.authorKrastev, DB-
dc.contributor.authorPettitt, SJ-
dc.contributor.authorCampbell, J-
dc.contributor.authorSong, F-
dc.contributor.authorTanos, BE-
dc.contributor.authorStoynov, SS-
dc.contributor.authorAshworth, A-
dc.contributor.authorLord, CJ-
dc.date.accessioned2022-01-17T12:46:45Z-
dc.date.available2018-05-22-
dc.date.available2022-01-17T12:46:45Z-
dc.date.issued2018-05-22-
dc.identifier2016-
dc.identifier.citationKrastev, D.B., Pettitt, S.J., Campbell, J., Song, F., Tanos, B.E., Stoynov, S.S., Ashworth, A. and Lord, C.J. (2018) 'Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets', Nature Communications, 9 (1), 2016, pp. 1-14. doi: 10.1038/s41467-018-04466-4.en_US
dc.identifier.urihttps://bura.brunel.ac.uk/handle/2438/23953-
dc.description.abstractCopyright © The Author(s) 2018. Poly (ADP-ribose)ylation is a dynamic protein modification that regulates multiple cellular processes. Here, we describe a system for identifying and characterizing PARylation events that exploits the ability of a PBZ (PAR-binding zinc finger) protein domain to bind PAR with high-affinity. By linking PBZ domains to bimolecular fluorescent complementation biosensors, we developed fluorescent PAR biosensors that allow the detection of temporal and spatial PARylation events in live cells. Exploiting transposon-mediated recombination, we integrate the PAR biosensor en masse into thousands of protein coding genes in living cells. Using these PAR-biosensor “tagged” cells in a genetic screen we carry out a large-scale identification of PARylation targets. This identifies CTIF (CBP80/CBP20-dependent translation initiation factor) as a novel PARylation target of the tankyrase enzymes in the centrosomal region of cells, which plays a role in the distribution of the centrosomal satellites.en_US
dc.description.sponsorshipBreast Cancer Now (BBC070X); Cancer Research UK through Program Grants (CRM059X).en_US
dc.format.extent1 - 14-
dc.format.mediumElectronic-
dc.languageEnglish-
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.rightsCopyright © The Author(s) 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectfluorescent proteinsen_US
dc.subjectpolyADP-ribosylationen_US
dc.titleCoupling bimolecular PARylation biosensors with genetic screens to identify PARylation targetsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1038/s41467-018-04466-4-
dc.relation.isPartOfNature Communications-
pubs.issue1-
pubs.publication-statusPublished-
pubs.volume9-
dc.identifier.eissn2041-1723-
Appears in Collections:Dept of Life Sciences Research Papers

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