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DC Field | Value | Language |
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dc.contributor.author | Madan, T | - |
dc.contributor.author | Biswas, B | - |
dc.contributor.author | Varghese, PM | - |
dc.contributor.author | Subedi, R | - |
dc.contributor.author | Pandit, H | - |
dc.contributor.author | Idicula-Thomas, S | - |
dc.contributor.author | Kundu, I | - |
dc.contributor.author | Rooge, S | - |
dc.contributor.author | Agarwal, R | - |
dc.contributor.author | Tripathi, DM | - |
dc.contributor.author | Kaur, S | - |
dc.contributor.author | Gupta, E | - |
dc.contributor.author | Gupta, SK | - |
dc.contributor.author | Kishore, U | - |
dc.date.accessioned | 2021-03-31T08:05:32Z | - |
dc.date.available | 2021-03-31T08:05:32Z | - |
dc.date.issued | 2021-03-30 | - |
dc.identifier.citation | Madan, T., Biswas, B., Varghese, P.M., Subedi, R., Pandit, H., Idicula-Thomas, S., Kundu, I., Rooge, S.B., Aggarwal, R., Tripathi, D., Kaur, S. (2021) 'A recombinant fragment of Human surfactant protein D binds Spike protein and inhibits infectivity and replication of SARS-CoV-2 in clinical samples', American Journal of Respiratory Cell and Molecular Biology, 65 (1), pp. 41-53. doi: 10.1165/rcmb.2021-0005OC. | en_US |
dc.identifier.issn | 1044-1549 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/22513 | - |
dc.description.abstract | COVID -19 is an acute infectious disease caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Human surfactant protein D (SP-D) is known to interact with spike protein of SARS-CoV, but its immune-surveillance against SARS-CoV-2 is not known. The study aimed to examine the potential of a recombinant fragment of human SP-D (rfhSP-D) as an inhibitor of replication and infection of SARS-CoV-2. The interaction of rfhSP-D with spike protein of SARS-CoV-2 and hACE-2 receptor was predicted via docking analysis. The inhibition of interaction between spike protein and ACE-2 by rfhSP-D was confirmed using direct and indirect ELISA. The effect of rfhSP-D on replication and infectivity of SARS-CoV-2 from clinical samples was studied by measuring the expression of RdRp gene of the virus using qPCR. In-silico interaction studies indicated that three amino acid residues in the RBD of spike of SARS-CoV-2 were commonly involved in interacting with rfhSP-D and ACE-2. Studies using clinical samples of SARS-CoV-2 positive cases (asymptomatic, n=7 and symptomatic, n=8 and negative controls n=15) demonstrated that treatment with 1.67 µM rfhSP-D inhibited viral replication by ~5.5 fold and was more efficient than Remdesivir (100 µM). Approximately, a 2-fold reduction in viral infectivity was also observed after treatment with 1.67 µM rfhSP-D. These results conclusively demonstrate that the calcium independent rfhSP-D mediated inhibition of binding between the receptor binding domain of the S1 subunit of the SARS-CoV-2 spike protein and human ACE-2, its host cell receptor, and a significant reduction in SARS-CoV-2 infection and replication in-vitro. | en_US |
dc.format.extent | 41 - 53 (13) | - |
dc.format.medium | Print-Electronic | - |
dc.language | English | - |
dc.language.iso | en | en_US |
dc.publisher | American Thoracic Society | en_US |
dc.rights | This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | surfactant protein D | en_US |
dc.subject | SARS-CoV-2 | en_US |
dc.subject | COVID-19 | en_US |
dc.subject | spike protein | en_US |
dc.subject | entry inhibition | en_US |
dc.title | A recombinant fragment of Human surfactant protein D binds Spike protein and inhibits infectivity and replication of SARS-CoV-2 in clinical samples | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1165/rcmb.2021-0005OC | - |
dc.relation.isPartOf | American Journal of Respiratory Cell and Molecular Biology | - |
pubs.issue | 1 | - |
pubs.publication-status | Published | - |
pubs.volume | 65 | - |
dc.identifier.eissn | 1535-4989 | - |
Appears in Collections: | Brunel Library Dept of Life Sciences Research Papers |
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