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DC Field | Value | Language |
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dc.contributor.author | Burnett, A | - |
dc.contributor.author | Gomez, I | - |
dc.contributor.author | De Leon, DD | - |
dc.contributor.author | Ariaans, M | - |
dc.contributor.author | Progias, P | - |
dc.contributor.author | Kammerer, RA | - |
dc.contributor.author | Velasco, G | - |
dc.contributor.author | Marron, M | - |
dc.contributor.author | Hellewell, P | - |
dc.contributor.author | Ridger, V | - |
dc.date.accessioned | 2020-01-20T14:50:52Z | - |
dc.date.available | 2017-12-01 | - |
dc.date.available | 2020-01-20T14:50:52Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Scientific Reports, 2017, 7 (1) | en_US |
dc.identifier.issn | http://dx.doi.org/10.1038/s41598-017-02216-y | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/20047 | - |
dc.description.abstract | Angiopoietins are a family of growth factors that are ligands for the tyrosine kinase receptor, Tie2. Angiopoietin 1 (Ang-1) is agonistic for Tie2, plays a key role in blood vessel maturation and stability and has been shown to possess anti-inflammatory properties. However, Tie2 expression has been demonstrated on human neutrophils and the observation that neutrophils migrate in response to Ang-1 in vitro has confounded research into its exact role in inflammation as well as its potential use as a therapeutic agent. We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. Tie2 expression was demonstrated on mouse neutrophils. In addition, recombinant human Ang-1 induced significant chemotaxis of isolated mouse neutrophils in a Tie2- and CD18-dependent manner. Subsequently, co-immunoprecipitation of Ang-1 and CD18 demonstrated their interaction. Intraperitoneal injection of an engineered angiopoietin-1, MAT.Ang-1, induced significant neutrophil migration into the peritoneum and a significant increase in the levels of CCL4 in peritoneal lavage fluid. Depletion of resident peritoneal macrophages prior to, or concomitant injections of an anti-CCL4 antibody with MAT.Ang-1 resulted in a significant reduction in neutrophil recruitment. These data indicate a pro-inflammatory role for Ang-1 with respect to neutrophil recruitment. | en_US |
dc.description.sponsorship | British Heart Foundation Studentship FS/06/081/21722 | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Research | en_US |
dc.title | Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4 | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1038/s41598-017-02216-y | - |
dc.relation.isPartOf | Scientific Reports | - |
pubs.issue | 1 | - |
pubs.publication-status | Published | - |
pubs.volume | 7 | - |
dc.identifier.eissn | 2045-2322 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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FullText.pdf | 1.5 MB | Adobe PDF | View/Open |
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