Once- and twice-daily heat acclimation confer similar heat adaptations, inflammatory responses and exercise tolerance improvements.

Abstract

This experiment aimed to investigate the efficacy of twice-daily, non-consecutive heat acclimation (TDHA) in comparison to once-daily heat acclimation (ODHA) and work matched once- or twice-daily temperate exercise (ODTEMP, TDTEMP) for inducing heat adaptations, improved exercise tolerance, and cytokine (immune) responses. Forty males, matched biophysically and for aerobic capacity, were assigned to ODHA, TDHA, ODTEMP or TDTEMP. Participants completed a cycling graded exercise test, heat acclimation state test and a time to task failure (TTTF) at 80% peak power output in temperate (TTTFTEMP: 22°C/40% RH) and hot conditions (TTTFHOT: 38°C/20% RH), before and after 10-sessions (60-min of cycling at ~2W.kg-1) in 45°C/20% RH (ODHA and TDHA) or 22°C/40% RH (ODTEMP or TDTEMP). Plasma IL-6, TNF-α and cortisol were measured pre- and post-sessions 1, 5 and 10. ODHA and TDHA induced equivalent heat adaptations (P<0.05) (resting rectal temperature [-0.28±0.22, -0.28±0.19°C], heart rate [-10±3, -10±4 b.min-1] and plasma volume expansion [+10.1±5.6, +8.5±3.1%]) and improved heat acclimation state (sweat setpoint [-0.22±0.18, -0.22±0.14°C] and gain [+0.14±0.10, +0.15±0.07g.sec-1.°C-1]). TTTFHOT increased (P<0.001) following ODHA (+25±4%) and TDHA (+24±10%), but not ODTEMP (+5±14%) or TDTEMP (+5±17%). TTTFTEMP did not improve (P>0.05) following ODHA (+14±4%), TDHA (14±8%), ODTEMP (9±10%) or TDTEMP (8±13%). Acute (P<0.05) but no chronic (P>0.05) increases were observed in IL-6, TNF-α or cortisol during ODHA and TDHA, or ODTEMP and TDTEMP. Once- and twice-daily heat acclimation conferred similar magnitudes of heat adaptation and exercise tolerance improvements, without differentially altering immune function, thus non-consecutive TDHA provides an effective, logistically flexible method of HA, benefitting individuals preparing for exercise-heat stress.