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http://bura.brunel.ac.uk/handle/2438/16961| Title: | Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry |
| Authors: | Larrouy-Maumus, G Clements, A Filloux, A McCarthy, RR Mostowy, S |
| Keywords: | Endotoxins;Mass spectrometry;Gram-negative bacteria |
| Issue Date: | 2015 |
| Publisher: | Elsevier |
| Citation: | Journal of Microbiological Methods, 2016, 120 pp. 68 - 71 |
| Abstract: | The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry. |
| URI: | http://bura.brunel.ac.uk/handle/2438/16961 |
| DOI: | http://dx.doi.org/10.1016/j.mimet.2015.12.004 |
| ISSN: | C C C C 0167-7012 http://dx.doi.org/10.1016/j.mimet.2015.12.004 http://dx.doi.org/10.1016/j.mimet.2015.12.004 |
| Other Identifiers: | C C |
| Appears in Collections: | Dept of Life Sciences Research Papers |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Fulltext.pdf | 463.97 kB | Adobe PDF | View/Open |
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