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DC Field | Value | Language |
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dc.contributor.author | de Castro, IJ | - |
dc.contributor.author | Amin, HA | - |
dc.contributor.author | Vinciotti, V | - |
dc.contributor.author | Vagnarelli, P | - |
dc.date.accessioned | 2017-08-31T13:05:51Z | - |
dc.date.available | 2017-08-31T13:05:51Z | - |
dc.date.issued | 2017-09-14 | - |
dc.identifier.citation | de Castro, I.J., Amin, H.A., Vinciotti, V. and Vagnarelli, P. (2017) 'Network of phosphatases and HDAC complexes at repressed chromatin', Cell Cycle, 16 (21), pp. 2011-2017. doi: 10.1080/15384101.2017.1371883. | en_US |
dc.identifier.issn | 1538-4101 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/15096 | - |
dc.description.abstract | © 2017 I. J. de Castro, H. A. Amin, V. Vinciotti, and P. Vagnarelli. Tight regulation of gene expression is achieved by a variety of protein complexes that selectively bind chromatin, modify it and change its transcription competency. Histone acetylases (HATs) and deacetylases (HDAC) play an important role in this process. They can generate transcriptionally active or inactive chromatin through the addition (HATs) or removal (HDACs) of acetyl groups on histones, respectively. Repo-Man is a Protein Phosphatase 1 targeting subunit that accumulates on chromosomes during mitotic exit and mediates the removal of mitotic histone H3 phosphorylations. It was shown recently that Repo-Man also regulates heterochromatin formation in interphase and that its depletion favours the switch between transcriptionally inactive and active chromatin, demonstrating that its role goes well beyond mitosis. Here, we provide the first link between a phosphatase and HDAC complexes. We show that genome-wide Repo-Man binding sites overlap with chromatin regions bound by members of the three HDAC complexes (Sin3a, NuRD and CoREST). We establish that members of the NuRD and Sin3a HDAC complexes interact with Repo-Man by mass spectrometry and that Repo-Man is in close proximity to Sap18 (Sin3a) in interphase by the Proximity Ligation Assay. Altogether, these data suggest a mechanism by which Repo-Man/PP1 complex, via interactions with HDACs, could stabilise gene repression | en_US |
dc.description.sponsorship | BBSRC grant BB/K017632/1 | - |
dc.format.extent | 2011 - 2017 | - |
dc.format.medium | Print-Electronic | - |
dc.language.iso | en | en_US |
dc.rights | © 2017 I. J. de Castro, H. A. Amin, V. Vinciotti, and P. Vagnarelli. Published with license by Taylor & Francis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | PP1 | - |
dc.subject | Repo-Man | - |
dc.subject | HDAC | - |
dc.subject | epigenetic | - |
dc.subject | heterochromatin | - |
dc.title | Network of phosphatases and HDAC complexes at repressed chromatin. | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1080/15384101.2017.1371883 | - |
dc.relation.isPartOf | Cell Cycle | - |
pubs.issue | 21 | - |
pubs.publication-status | Published | - |
pubs.volume | 16 | - |
dc.identifier.eissn | 1551-4005 | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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