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http://bura.brunel.ac.uk/handle/2438/796
Title: | Comparison of Short-Term Estrogenicity Tests for Identification of Hormone-Disrupting Chemicals |
Authors: | Andersen, HR Andersson, AM Arnold, SF Autrup, H Barfoed, N Beresford, NA Bjerregaard, P Christiansen, LB Gissel, B Hummel, R Jorgensen, EB Korsgaard, B Le Guevel, R Leffers, H McLachlan, J Moller, A Nielsen, JB Olea, N Oles-Karasko, A Pakdel, F Pedersen, KL Perez, P Skakkeboek, NE Sonnenschein, C Soto, AM Sumpter, JP Thorpe, SM Grandjean, P |
Keywords: | Estrogenic chemicals;Estrogens;Antiestrogens;Estrogenicity tests;Binding assay;Yeast;MCF-7;Vitellogenin |
Issue Date: | 1999 |
Publisher: | National Institute of Environmental Health Science |
Citation: | Environ Health Perspect. 1999 Feb; 107(Suppl 1): 89-108 |
Abstract: | The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods. |
URI: | http://bura.brunel.ac.uk/handle/2438/796 |
Appears in Collections: | Institute for the Environment |
Files in This Item:
File | Description | Size | Format | |
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Comparison of short-term Estrogenicity.pdf | 4.04 MB | Adobe PDF | View/Open |
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