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Title: | Comparison of short-term estrogenicity tests for identification of hormone-disrupting chemicals |
Authors: | Andersen, HR Andersson, AM Arnold, SF Autrup, H Barfoed, M Beresford, NA Bjerregaard, P Christiansen, LB Gissel, B Hummel, R Jorgensen, EB Korsgaard, B Le Guevel, R Leffers, H McLachlan, J Moller, A Nielsen, JB Olea, N Oles-Karasko, A Pakdel, F Pedersen, KL Perez, P Skakkeboek, NE Sonnenschein, C Soto, AM Sumpter, JP Thorpe, SM Grandjean, P |
Keywords: | Estrogenic chemicals;Estrogens;Antiestrogens;Estrogenicity tests;Binding assay;Yeast;MCF-7;Vitellogenin |
Issue Date: | 1999 |
Publisher: | National Institute of Environmental Health Sciences (NIEHS) |
Citation: | Environmental Health Perspectives, 107: 89 - 108, Feb 1999 |
Abstract: | The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the lest panel were 17 beta-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17 beta-Estradioi. 17 alpha-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all rest systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds-tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate. 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate. dibutylphthalate, methoxychlor, o,p'-DDT, p,p'-DDE, endosulfan, chlomequat chloride, and ethanol-varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods. |
Description: | Copyright @ 1999 The National Institute of Environmental Health Sciences (NIEHS) |
URI: | http://www.jstor.org/stable/3434476 http://bura.brunel.ac.uk/handle/2438/5543 |
ISSN: | 0091-6765 |
Appears in Collections: | Publications |
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Fulltext.pdf | 2.21 MB | Adobe PDF | View/Open |
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