Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/8821
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DC Field | Value | Language |
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dc.contributor.author | Merryweather-Clarke, AT | - |
dc.contributor.author | Atzberger, A | - |
dc.contributor.author | Soneji, S | - |
dc.contributor.author | Gray, N | - |
dc.contributor.author | Clark, K | - |
dc.contributor.author | Waugh, C | - |
dc.contributor.author | McGowan, SJ | - |
dc.contributor.author | Taylor, S | - |
dc.contributor.author | Nandi, AK | - |
dc.contributor.author | Wood, WG | - |
dc.contributor.author | Roberts, DJ | - |
dc.contributor.author | Higgs, DR | - |
dc.contributor.author | Buckle, VJ | - |
dc.contributor.author | Robson, KJH | - |
dc.date.accessioned | 2014-08-04T12:45:26Z | - |
dc.date.available | 2014-08-04T12:45:26Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Blood, 117(13), e96-e108, 2011 | en_US |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.uri | http://bloodjournal.hematologylibrary.org/content/117/13/e96 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/8821 | - |
dc.description | This article is available open access through the publisher’s website. Copyright @ 2011 American Society of Hematology. This article has an erratum: http://bloodjournal.hematologylibrary.org/content/118/26/6993.3. | en_US |
dc.description.abstract | Understanding the pattern of gene expression during erythropoiesis is crucial for a synthesis of erythroid developmental biology. Here, we isolated 4 distinct populations at successive erythropoietin-dependent stages of erythropoiesis, including the terminal, pyknotic stage. The transcriptome was determined using Affymetrix arrays. First, we demonstrated the importance of using defined cell populations to identify lineage and temporally specific patterns of gene expression. Cells sorted by surface expression profile not only express significantly fewer genes than unsorted cells but also demonstrate significantly greater differences in the expression levels of particular genes between stages than unsorted cells. Second, using standard software, we identified more than 1000 transcripts not previously observed to be differentially expressed during erythroid maturation, 13 of which are highly significantly terminally regulated, including RFXAP and SMARCA4. Third, using matched filtering, we identified 12 transcripts not previously reported to be continuously up-regulated in maturing human primary erythroblasts. Finally, using transcription factor binding site analysis, we identified potential transcription factors that may regulate gene expression during terminal erythropoiesis. Our stringent lists of differentially regulated and continuously expressed transcripts containing many genes with undiscovered functions in erythroblasts are a resource for future functional studies of erythropoiesis. Our Human Erythroid Maturation database is available at https://cellline.molbiol.ox.ac.uk/eryth/index.html. | en_US |
dc.description.sponsorship | National Health Service Blood and Transplant, National Institute for Health Research Biomedical Research Center Program, and National Institute for Health Research. | en_US |
dc.language | English | - |
dc.language.iso | en | en_US |
dc.publisher | American Society of Hematology | en_US |
dc.subject | Gene expression | en_US |
dc.subject | Erythropoiesis | en_US |
dc.subject | Human erythroid progenitors | en_US |
dc.subject | Cell populations | en_US |
dc.title | Global gene expression analysis of human erythroid progenitors | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1182/blood-2010-07-290825 | - |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Electronic and Computer Engineering | - |
pubs.organisational-data | /Brunel/Brunel Staff by College/Department/Division/College of Engineering, Design and Physical Sciences/Dept of Electronic and Computer Engineering/Electronic and Computer Engineering | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/Brunel Business School - URCs and Groups/Centre for Research into Entrepreneurship, International Business and Innovation in Emerging Markets | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute for Ageing Studies | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Brunel Institute of Cancer Genetics and Pharmacogenomics | - |
pubs.organisational-data | /Brunel/University Research Centres and Groups/School of Health Sciences and Social Care - URCs and Groups/Centre for Systems and Synthetic Biology | - |
Appears in Collections: | Electronic and Computer Engineering Dept of Electronic and Electrical Engineering Research Papers |
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Notice.pdf | 42.01 kB | Adobe PDF | View/Open |
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