Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/8557
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dc.contributor.authorTakagi, M-
dc.contributor.authorSanchez-Pulido, L-
dc.contributor.authorPetfalski, E-
dc.contributor.authorVargiu, G-
dc.contributor.authorSamejima, K-
dc.contributor.authorImamoto, N-
dc.contributor.authorPonting, CP-
dc.contributor.authorTollervey, D-
dc.contributor.authorEarnshaw, WC-
dc.contributor.authorVagnarelli, P-
dc.date.accessioned2014-06-05T12:50:38Z-
dc.date.available2014-06-05T12:50:38Z-
dc.date.issued2014-
dc.identifier.citationElife, 3: e01641 22 pp, May 2014en_US
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/24867636en
dc.identifier.urihttp://bura.brunel.ac.uk/handle/2438/8557-
dc.descriptionCopyright @ 2014 Booth et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en_US
dc.description.abstractWhen the nucleolus disassembles during open mitosis, many nucleolar proteins and RNAs associate with chromosomes, establishing a perichromosomal compartment coating the chromosome periphery. At present nothing is known about the function of this poorly characterised compartment. In this study, we report that the nucleolar protein Ki-67 is required for the assembly of the perichromosomal compartment in human cells. Ki-67 is a cell-cycle regulated protein phosphatase 1-binding protein that is involved in phospho-regulation of the nucleolar protein B23/nucleophosmin. Following siRNA depletion of Ki-67, NIFK, B23, nucleolin, and four novel chromosome periphery proteins all fail to associate with the periphery of human chromosomes. Correlative light and electron microscopy (CLEM) images suggest a near-complete loss of the entire perichromosomal compartment. Mitotic chromosome condensation and intrinsic structure appear normal in the absence of the perichromosomal compartment but significant differences in nucleolar reassembly and nuclear organisation are observed in post-mitotic cells.en_US
dc.languageeng-
dc.language.isoenen_US
dc.publishereLife Sciences Publications Ltden_US
dc.subjectChromosomesen_US
dc.subjectMitosisen_US
dc.subjectNucleolusen_US
dc.subjectPhosphatasesen_US
dc.titleKi-67 is a PP1-interacting protein that organises the mitotic chromosome peripheryen_US
dc.typeArticleen_US
dc.identifier.doihttp://dx.doi.org/10.7554/eLife.01641-
pubs.organisational-data/Brunel-
pubs.organisational-data/Brunel/Brunel Active Staff-
pubs.organisational-data/Brunel/Brunel Active Staff/School of Health Sciences & Social Care-
pubs.organisational-data/Brunel/Brunel Active Staff/School of Health Sciences & Social Care/Biological Sciences-
Appears in Collections:Biological Sciences
Dept of Life Sciences Research Papers

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