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http://bura.brunel.ac.uk/handle/2438/796
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DC Field | Value | Language |
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dc.contributor.author | Andersen, HR | - |
dc.contributor.author | Andersson, AM | - |
dc.contributor.author | Arnold, SF | - |
dc.contributor.author | Autrup, H | - |
dc.contributor.author | Barfoed, N | - |
dc.contributor.author | Beresford, NA | - |
dc.contributor.author | Bjerregaard, P | - |
dc.contributor.author | Christiansen, LB | - |
dc.contributor.author | Gissel, B | - |
dc.contributor.author | Hummel, R | - |
dc.contributor.author | Jorgensen, EB | - |
dc.contributor.author | Korsgaard, B | - |
dc.contributor.author | Le Guevel, R | - |
dc.contributor.author | Leffers, H | - |
dc.contributor.author | McLachlan, J | - |
dc.contributor.author | Moller, A | - |
dc.contributor.author | Nielsen, JB | - |
dc.contributor.author | Olea, N | - |
dc.contributor.author | Oles-Karasko, A | - |
dc.contributor.author | Pakdel, F | - |
dc.contributor.author | Pedersen, KL | - |
dc.contributor.author | Perez, P | - |
dc.contributor.author | Skakkeboek, NE | - |
dc.contributor.author | Sonnenschein, C | - |
dc.contributor.author | Soto, AM | - |
dc.contributor.author | Sumpter, JP | - |
dc.contributor.author | Thorpe, SM | - |
dc.contributor.author | Grandjean, P | - |
dc.coverage.spatial | 20 | en |
dc.date.accessioned | 2007-05-24T14:03:34Z | - |
dc.date.available | 2007-05-24T14:03:34Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Environ Health Perspect. 1999 Feb; 107(Suppl 1): 89-108 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/796 | - |
dc.description.abstract | The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the test panel were 17β-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17β-Estradiol, 17α-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all test systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds—tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate, 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate, dibutylphthalate, methoxychlor, o,p′-DDT, p,p′-DDE, endosulfan, chlomequat chloride, and ethanol—varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods. | en |
dc.format.extent | 4137670 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | National Institute of Environmental Health Science | en |
dc.subject | Estrogenic chemicals | en |
dc.subject | Estrogens | en |
dc.subject | Antiestrogens | en |
dc.subject | Estrogenicity tests | en |
dc.subject | Binding assay | en |
dc.subject | Yeast | en |
dc.subject | MCF-7 | en |
dc.subject | Vitellogenin | en |
dc.title | Comparison of Short-Term Estrogenicity Tests for Identification of Hormone-Disrupting Chemicals | en |
dc.type | Research Paper | en |
Appears in Collections: | Institute for the Environment |
Files in This Item:
File | Description | Size | Format | |
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Comparison of short-term Estrogenicity.pdf | 4.04 MB | Adobe PDF | View/Open |
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