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DC Field | Value | Language |
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dc.contributor.author | Andersen, HR | - |
dc.contributor.author | Andersson, AM | - |
dc.contributor.author | Arnold, SF | - |
dc.contributor.author | Autrup, H | - |
dc.contributor.author | Barfoed, M | - |
dc.contributor.author | Beresford, NA | - |
dc.contributor.author | Bjerregaard, P | - |
dc.contributor.author | Christiansen, LB | - |
dc.contributor.author | Gissel, B | - |
dc.contributor.author | Hummel, R | - |
dc.contributor.author | Jorgensen, EB | - |
dc.contributor.author | Korsgaard, B | - |
dc.contributor.author | Le Guevel, R | - |
dc.contributor.author | Leffers, H | - |
dc.contributor.author | McLachlan, J | - |
dc.contributor.author | Moller, A | - |
dc.contributor.author | Nielsen, JB | - |
dc.contributor.author | Olea, N | - |
dc.contributor.author | Oles-Karasko, A | - |
dc.contributor.author | Pakdel, F | - |
dc.contributor.author | Pedersen, KL | - |
dc.contributor.author | Perez, P | - |
dc.contributor.author | Skakkeboek, NE | - |
dc.contributor.author | Sonnenschein, C | - |
dc.contributor.author | Soto, AM | - |
dc.contributor.author | Sumpter, JP | - |
dc.contributor.author | Thorpe, SM | - |
dc.contributor.author | Grandjean, P | - |
dc.date.accessioned | 2011-07-11T11:15:49Z | - |
dc.date.available | 2011-07-11T11:15:49Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Environmental Health Perspectives, 107: 89 - 108, Feb 1999 | en_US |
dc.identifier.issn | 0091-6765 | - |
dc.identifier.uri | http://www.jstor.org/stable/3434476 | en |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/5543 | - |
dc.description | Copyright @ 1999 The National Institute of Environmental Health Sciences (NIEHS) | en_US |
dc.description.abstract | The aim of this study was to compare results obtained by eight different short-term assays of estrogenlike actions of chemicals conducted in 10 different laboratories in five countries. Twenty chemicals were selected to represent direct-acting estrogens, compounds with estrogenic metabolites, estrogenic antagonists, and a known cytotoxic agent. Also included in the lest panel were 17 beta-estradiol as a positive control and ethanol as solvent control. The test compounds were coded before distribution. Test methods included direct binding to the estrogen receptor (ER), proliferation of MCF-7 cells, transient reporter gene expression in MCF-7 cells, reporter gene expression in yeast strains stably transfected with the human ER and an estrogen-responsive reporter gene, and vitellogenin production in juvenile rainbow trout. 17 beta-Estradioi. 17 alpha-ethynyl estradiol, and diethylstilbestrol induced a strong estrogenic response in all rest systems. Colchicine caused cytotoxicity only. Bisphenol A induced an estrogenic response in all assays. The results obtained for the remaining test compounds-tamoxifen, ICI 182.780, testosterone, bisphenol A dimethacrylate. 4-n-octylphenol, 4-n-nonylphenol, nonylphenol dodecylethoxylate, butylbenzylphthalate. dibutylphthalate, methoxychlor, o,p'-DDT, p,p'-DDE, endosulfan, chlomequat chloride, and ethanol-varied among the assays. The results demonstrate that careful standardization is necessary to obtain a reasonable degree of reproducibility. Also, similar methods vary in their sensitivity to estrogenic compounds. Thus, short-term tests are useful for screening purposes, but the methods must be further validated by additional interlaboratory and interassay comparisons to document the reliability of the methods. | en_US |
dc.language | EN | - |
dc.language.iso | en | en_US |
dc.publisher | National Institute of Environmental Health Sciences (NIEHS) | en_US |
dc.subject | Estrogenic chemicals | en_US |
dc.subject | Estrogens | en_US |
dc.subject | Antiestrogens | en_US |
dc.subject | Estrogenicity tests | en_US |
dc.subject | Binding assay | en_US |
dc.subject | Yeast | en_US |
dc.subject | MCF-7 | en_US |
dc.subject | Vitellogenin | en_US |
dc.title | Comparison of short-term estrogenicity tests for identification of hormone-disrupting chemicals | en_US |
dc.type | Article | en_US |
pubs.organisational-data | /Brunel | - |
pubs.organisational-data | /Brunel/Brunel (Active) | - |
pubs.organisational-data | /Brunel/Brunel (Active)/Institute for the Environment | - |
pubs.organisational-data | /Brunel/Brunel (Active)/Research Centres | - |
pubs.organisational-data | /Brunel/Institute for the Environment | - |
pubs.organisational-data | /Brunel/Research Centres | - |
Appears in Collections: | Publications |
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Fulltext.pdf | 2.21 MB | Adobe PDF | View/Open |
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