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DC Field | Value | Language |
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dc.contributor.author | Gibson, O | - |
dc.contributor.author | Astin, R | - |
dc.contributor.author | Puthucheary, Z | - |
dc.contributor.author | Preston, S | - |
dc.contributor.author | Yadav, S | - |
dc.contributor.author | Gavins, F | - |
dc.contributor.author | González-Alonso, J | - |
dc.date.accessioned | 2022-12-10T18:10:12Z | - |
dc.date.available | 2022-12-10T18:10:12Z | - |
dc.date.issued | 2022-12-13 | - |
dc.identifier | ORCID iDs: Oliver R. Gibson https://orcid.org/0000-0001-6777-5562; Shreya Yadav https://orcid.org/0000-0002-1428-4847; Felicity N.E. Gavins https://orcid.org/0000-0001-7008-5423; José González-Alonso https://orcid.org/0000-0002-8205-3311. | - |
dc.identifier.citation | Gibson, O.R. et al. (2023) 'Skeletal muscle angiogenic, regulatory and heat shock protein responses to prolonged passive hyperthermia of the human lower limb', American Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 324 (1), pp. R1 - R14. doi: 10.1152/ajpregu.00320.2021. | en_US |
dc.identifier.issn | 0363-6119 | - |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/25617 | - |
dc.description.abstract | Passive hyperthermia induces a range of physiological responses including augmenting skeletal muscle mRNA expression. This experiment aimed to examine gene and protein responses to prolonged passive leg hyperthermia. Seven young participants underwent 3 h of resting unilateral leg heating (HEAT) followed by a further 3 h of rest, with the contralateral leg serving as an unheated control (CONT). Muscle biopsies were taken at baseline (0 h), and 1.5, 3, 4, and 6 h in HEAT and 0 and 6 h in CONT to assess changes in selected mRNA expression via qRT-PCR, and HSP72 and VEGFα concentration via ELISA. Muscle temperature (Tm) increased in HEAT plateauing from 1.5 to 3 h (+3.5±1.5°C from 34.2±1.2°C baseline value; p<0.001), returning to baseline at 6 h. No change occurred in CONT. eNOS, FOXO-1, Hsp72, and VEGFα mRNA increased in HEAT (p<0.05) however post-hoc analysis identified that only Hsp72 mRNA statistically increased (at 4 h vs. baseline). When peak change during HEAT was calculated ANGPT-2 decreased (-0.4±0.2-fold), and CCL2 (+2.9±1.6-fold), FOXO-1 (+6.2±4.4-fold), Hsp27 (+2.9±1.7-fold), Hsp72 (+8.5±3.5-fold), Hsp90α (+4.6±3.7-fold), and VEGFα (+5.9±3.1-fold) increased from baseline (all p<0.05). At 6 h Tm were not different between limbs (p=0.582; CONT=32.5±1.6°C, HEAT=34.3±1.2°C), and only ANGPT-2 (p=0.031;-1.3±1.4-fold) and VEGFα (p=0.030;1.1±1.2-fold) differed between HEAT and CONT. No change in VEGFα or HSP72 protein concentration were observed over time, however, peak change in VEGFα did increase (p<0.05) in HEAT (+140±184 pg.mL-1) vs CONT (+7±86 pg.mL-1). Passive hyperthermia transiently augmented ANGPT-2, CCL2, eNOS, FOXO-1, Hsp27, Hsp72, Hsp90α and VEGFα mRNA, and VEGFα protein. | en_US |
dc.description.sponsorship | The Physiological Society’s 2017 Early Career Research Grant scheme, Brunel University London’s Research Initiative and Enterprise Fund (BRIEF, 2017), and the Centre for Human Performance, Exercise and Rehabilitation (CHPER), Centre for Physical Activity in Health and Disease (CHPAD), at Brunel University London. | en_US |
dc.format.extent | R1 - R14 | - |
dc.format.medium | Print-Electronic | - |
dc.language.iso | en_US | en_US |
dc.publisher | American Physiological Society | en_US |
dc.rights | Copyright © 2022 American Physiological Society. This is the author’s version of the work. It is posted here by permission of the APS for personal use, not for redistribution. The definitive version was published in American Journal of Physiology: Regulatory, Integrative and Comparative Physiology Journal, 0 (in press) published online 21 November 2022, DOI: 10.1152/ajpregu.00320.2021 | - |
dc.rights.uri | https://journals.physiology.org/author-info.permissions | - |
dc.subject | angiogenesis | en_US |
dc.subject | heat shock proteins | en_US |
dc.subject | heat therapy | en_US |
dc.subject | hyperthermia | en_US |
dc.subject | passive heating | en_US |
dc.title | Skeletal muscle angiogenic, regulatory and heat shock protein responses to prolonged passive hyperthermia of the human lower limb | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1152/ajpregu.00320.2021 | - |
dc.relation.isPartOf | American Journal of Physiology: Regulatory, Integrative and Comparative Physiology | - |
pubs.issue | 1 | - |
pubs.publication-status | Published | - |
pubs.volume | 324 | - |
dc.identifier.eissn | 1522-1490 | - |
dc.rights.holder | American Physiological Society | - |
Appears in Collections: | Dept of Life Sciences Embargoed Research Papers |
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FullText.pdf | Embargoed until 13 December 2023 | 878.41 kB | Adobe PDF | View/Open |
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