Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/23506
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yin, S | - |
dc.contributor.author | Liu, Y | - |
dc.contributor.author | Dai, S | - |
dc.contributor.author | Zhang, B | - |
dc.contributor.author | Qu, Y | - |
dc.contributor.author | Zhang, Y | - |
dc.contributor.author | Choe, W-S | - |
dc.contributor.author | Bi, J | - |
dc.date.accessioned | 2021-11-13T22:32:48Z | - |
dc.date.available | 2021-11-13T22:32:48Z | - |
dc.date.issued | 2021-11-11 | - |
dc.identifier | ORCiD: Sheng Dai https://orcid.org/0000-0002-9152-0786 | - |
dc.identifier | ORCiD: Yao Zhang https://orcid.org/0000-0002-0776-1764 | - |
dc.identifier | 444 | - |
dc.identifier.citation | Yin, S. et al. (2021) ‘Mechanism Study of Thermally Induced Anti-Tumor Drug Loading to Engineered Human Heavy-Chain Ferritin Nanocages Aided by Computational Analysis’, Biosensors, 11 (11), 444, pp. 1 - 17. doi: 10.3390/bios11110444. | en_US |
dc.identifier.uri | https://bura.brunel.ac.uk/handle/2438/23506 | - |
dc.description | Data Availability Statement: The data presented in this study are available on request from the corresponding author. | - |
dc.description | Supplementary Materials are available online at: https://doi.org/10.3390/bios11110444 . | - |
dc.description.abstract | Diverse drug loading approaches for human heavy-chain ferritin (HFn), a promising drug nanocarrier, have been established. However, anti-tumor drug loading ratio and protein carrier recovery yield are bottlenecks for future clinical application. Mechanisms behind drug loading have not been elaborated. In this work, a thermally induced drug loading approach was introduced to load anti-tumor drug doxorubicin hydrochloride (DOX) into HFn, and 2 functionalized HFns, HFn-PAS-RGDK, and HFn-PAS. Optimal conditions were obtained through orthogonal tests. All 3 HFn-based proteins achieved high protein recovery yield and drug loading ratio. Size exclusion chromatography (SEC) and transmission electron microscopy (TEM) results showed the majority of DOX loaded protein (protein/DOX) remained its nanocage conformation. Computational analysis, molecular docking followed by molecular dynamic (MD) simulation, revealed mechanisms of DOX loading and formation of by-product by investigating non-covalent interactions between DOX with HFn subunit and possible binding modes of DOX and HFn after drug loading. In in vitro tests, DOX in protein/DOX entered tumor cell nucleus and inhibited tumor cell growth. | en_US |
dc.description.sponsorship | joint Ph.D. Scholarship Scheme of the University of Adelaide and Institute of Process Engineering, Chinese Academy of Sciences, the National Natural Science Foundation of China [Grant No. 21576267]; Beijing Natural Science Foundation [Grant Number 2162041]. | en_US |
dc.format.extent | 1 - 17 | - |
dc.format.medium | Electronic | - |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.rights | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | ferritin | en_US |
dc.subject | drug delivery | en_US |
dc.subject | thermally induced drug loading | en_US |
dc.subject | computational analysis | en_US |
dc.title | Mechanism Study of Thermally Induced Anti-Tumor Drug Loading to Engineered Human Heavy-Chain Ferritin Nanocages Aided by Computational Analysis | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.3390/bios11110444 | - |
dc.relation.isPartOf | Biosensors | - |
pubs.issue | 11 | - |
pubs.publication-status | Published online | - |
pubs.volume | 11 | - |
dc.identifier.eissn | 2079-6374 | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/legalcode.en | - |
dc.rights.holder | The authors | - |
Appears in Collections: | Dept of Chemical Engineering Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
FullText.pdf | Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | 4.98 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License