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DC Field | Value | Language |
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dc.contributor.author | de Souza Figueiredo, F | - |
dc.contributor.author | Celano, R | - |
dc.contributor.author | de Sousa Silva, D | - |
dc.contributor.author | das Neves Costa, F | - |
dc.contributor.author | Hewitson, P | - |
dc.contributor.author | Ignatova, S | - |
dc.contributor.author | Piccinelli, AL | - |
dc.contributor.author | Rastrelli, L | - |
dc.contributor.author | Guimarães Leitão, S | - |
dc.contributor.author | Guimarães Leitão, G | - |
dc.date.accessioned | 2017-03-15T14:01:54Z | - |
dc.date.available | 2016-12-19 | - |
dc.date.available | 2017-03-15T14:01:54Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Journal of Chromatography A, 1481: pp. 92–100, (2017) | en_US |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/14252 | - |
dc.description.abstract | Ampelozizyphus amazonicus Ducke (Rhamnaceae), a medicinal plant used to prevent malaria, is a climbing shrub, native to the Amazonian region, with jujubogenin glycoside saponins as main compounds. The crude extract of this plant is too complex for any kind of structural identification, and HPLC separation was not sufficient to resolve this issue. Therefore, the aim of this work was to obtain saponin enriched fractions from the bark ethanol extract by countercurrent chromatography (CCC) for further isolation and identification/characterisation of the major saponins by HPLC and MS. The butanol extract was fractionated by CCC with hexane - ethyl acetate - butanol - ethanol - water (1:6:1:1:6; v/v) solvent system yielding 4 group fractions. The collected fractions were analysed by UHPLC-HRMS (ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry) and MSn. Group 1 presented mainly oleane type saponins, and group 3 showed mainly jujubogenin glycosides, keto-dammarane type triterpene saponins and saponins with C31 skeleton. Thus, CCC separated saponins from the butanol-rich extract by skeleton type. A further purification of group 3 by CCC (ethyl acetate - ethanol - water (1:0.2:1; v/v)) and HPLC-RI was performed in order to obtain these unusual aglycones in pure form. | en_US |
dc.description.sponsorship | F.S. Figueiredo is indebted to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for the Ph.D scholarship. F.N. Costa and S. Ignatova would like to thank Newton Advanced Fellowship project funded by the Royal Society of the United Kingdom. S.G. Leitão and G.G. Leitão are indebted to FAPERJ and CNPq for financial support. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Ampelozizyphus amazonicus | en_US |
dc.subject | Rhamnaceae | en_US |
dc.subject | Saponin skeleton type | en_US |
dc.subject | Triterpene saponins | en_US |
dc.subject | Countercurrent chromatography | en_US |
dc.subject | HPLC–MS | en_US |
dc.title | Countercurrent chromatography separation of saponins by skeleton type from Ampelozizyphus amazonicus for off-line ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry analysis and characterisation | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.1016/j.chroma.2016.12.053 | - |
dc.relation.isPartOf | Journal of chromatography. A | - |
pubs.publication-status | Published | - |
Appears in Collections: | Dept of Life Sciences Research Papers |
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