Please use this identifier to cite or link to this item: http://bura.brunel.ac.uk/handle/2438/13928
Title: Cytokine therapy-mediated neuroprotection in a Friedreich's ataxia mouse model
Authors: Kemp, KC
Cerminara, N
Hares, K
Redondo, J
Cook, AJ
Haynes, HR
Burton, BR
Pook, M
Apps, R
Scolding, NJ
Wilkins, A
Issue Date: 23-Dec-2016
Citation: Kemp KC, Cerminara N, Hares K, Redondo J, Cook AJ, Haynes HR, Burton BR, Pook M, Apps R, Scolding NJ, Wilkins A. Cytokine therapy‐mediated neuroprotection in a Friedreich's ataxia mouse model. Annals of Neurology. 2017 Feb;81(2):212-26.
Abstract: Objectives Friedreich's ataxia is a devastating neurological disease currently lacking any proven treatment. We studied the neuroprotective effects of the cytokines, granulocyte‐colony stimulating factor (G‐CSF) and stem cell factor (SCF) in a humanized murine model of Friedreich's ataxia. Methods Mice received monthly subcutaneous infusions of cytokines while also being assessed at monthly time points using an extensive range of behavioral motor performance tests. After 6 months of treatment, neurophysiological evaluation of both sensory and motor nerve conduction was performed. Subsequently, mice were sacrificed for messenger RNA, protein, and histological analysis of the dorsal root ganglia, spinal cord, and cerebellum. Results Cytokine administration resulted in significant reversal of biochemical, neuropathological, neurophysiological, and behavioural deficits associated with Friedreich's ataxia. Both G‐CSF and SCF had pronounced effects on frataxin levels (the primary molecular defect in the pathogenesis of the disease) and a regulators of frataxin expression. Sustained improvements in motor coordination and locomotor activity were observed, even after onset of neurological symptoms. Treatment also restored the duration of sensory nerve compound potentials. Improvements in peripheral nerve conduction positively correlated with cytokine‐induced increases in frataxin expression, providing a link between increases in frataxin and neurophysiological function. Abrogation of disease‐related pathology was also evident, with reductions in inflammation/gliosis and increased neural stem cell numbers in areas of tissue injury. Interpretation These experiments show that cytokines already clinically used in other conditions offer the prospect of a novel, rapidly translatable, disease‐modifying, and neuroprotective treatment for Friedreich's ataxia.
URI: http://bura.brunel.ac.uk/handle/2438/13928
DOI: http://dx.doi.org/10.1002/ana.24846
ISSN: 0364-5134
Appears in Collections:Dept of Life Sciences Research Papers

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