Please use this identifier to cite or link to this item:
http://bura.brunel.ac.uk/handle/2438/11037
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mouratidis, PX | - |
dc.contributor.author | George, AJ | - |
dc.coverage.spatial | New Zealand | - |
dc.coverage.spatial | New Zealand | - |
dc.date.accessioned | 2015-06-22T12:20:23Z | - |
dc.date.available | 2015 | - |
dc.date.available | 2015-06-22T12:20:23Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Journal of Inflammation Research, 2015: 8, 97–106, (21 May 2015) | en_US |
dc.identifier.issn | 1178-7031 | - |
dc.identifier.uri | http://www.dovepress.com/regulation-of-indoleamine-23-dioxygenase-in-primary-human-saphenous-ve-peer-reviewed-article-JIR | - |
dc.identifier.uri | http://bura.brunel.ac.uk/handle/2438/11037 | - |
dc.description.abstract | BACKGROUND: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior. METHODS: IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay. RESULTS: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ but not after treatment with TNFα, IL-1β, IL-2, IL-4, IL-6, or IL-10. VEGFβ and heparin negatively regulate IFNγ-driven increases in IDO. Overexpression of IDO in endothelial cells does not affect transmigration of T-cells. CONCLUSION: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ. Heparin and angiogenesis stimulators such as VEGFβ negatively regulate its expression. | en_US |
dc.description.sponsorship | The British Heart Foundation (BHF). | en_US |
dc.format.extent | 97 - 106 | - |
dc.language | eng | - |
dc.language.iso | en | en_US |
dc.publisher | Dove Press Ltd | en_US |
dc.subject | HSVEC | en_US |
dc.subject | IFNγ | en_US |
dc.subject | VEGFβ | en_US |
dc.subject | Endothelium | en_US |
dc.subject | Inflammation | en_US |
dc.title | Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells. | en_US |
dc.type | Article | en_US |
dc.identifier.doi | http://dx.doi.org/10.2147/JIR.S82202 | - |
dc.relation.isPartOf | J Inflamm Res | - |
pubs.volume | 8 | - |
pubs.volume | 8 | - |
Appears in Collections: | Dept of Health Sciences Research Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Fulltext.pdf | 756.16 kB | Adobe PDF | View/Open |
Items in BURA are protected by copyright, with all rights reserved, unless otherwise indicated.